The year 2003 will celebrate two anniversaries of some significance to geneticists. The first of these – the publication of Watson and Crick's paper on the structure of DNA back in 1953 – will ...

A technology that uses enzymes to cut and paste together DNA sequences of interest. The recombined DNA sequences can be placed into vectors that carry the DNA into a host cell. In this host cell, the ...

Scientists at Johns Hopkins, Rutgers, the University of Trento in Italy, and Harvard Medical School report they have developed a new molecular technique that can be used to isolate thousands of long ...

One of the most indispensable techniques in all areas of the life sciences is the use of expression vectors. Cloning a gene between two restriction sites, and the expression of a protein, are almost ...

Clyde A. Hutchison III, Hamilton O. Smith, Cynthia Pfannkoch, J. Craig Venter, Cell-Free Cloning Using ϕ29 DNA Polymerase, Proceedings of the National Academy of Sciences of the United States of ...

Molecular biology has transitioned to a new era where precise genetic manipulation plays a critical role in research, medicine, and biotechnology. Among the vital tools in this field are cloning ...

Cell-Free DNA Cloning Cell-based methods for cloning DNA that rely on E. coli are time-consuming and inefficient. OriCiro has developed new DNA cloning techniques that bypass E. coli completely ...

This cloning-free method involves the insertion of long DNA fragments in two steps. In the first step, a small portion (less than 200 nucleotides) of the long fragment is inserted in the genome.

The black-footed ferret was believed extinct until 18 were discovered on a Wyoming ranch. Now scientists have cloned one using 33-year-old DNA.