PCR——实验室必备实验,从基因鉴定到信号通路验证,几乎每周都要做那么几次。 然而,各种PCR技术,包括PCR、qPCR、RT-PCR、RT-qPCR、Real-Time PCR和dPCR,长得实在是太像了,好多同学看得头晕眼花也没弄明白这几种PCR有什么区别。 今天,一篇文章,带你清清楚楚 ...
Polymerase chain reaction (PCR) is a technique that has revolutionized the world of molecular biology and beyond, enabling the amplification of nucleotide sequences. In this article, we will discuss a ...
In 1983, American biochemist Kary Mullis was driving home late at night when a flash of inspiration struck him. He wrote on the back of a receipt the idea that would eventually grant him the Nobel ...
Analyzing DNA is useful for a number of vital applications. This includes diagnosis and monitoring of diseases, identification of criminals, and studying the function of a targeted segment of DNA.
Since its original development (2008) COLD-PCR has been used by several groups for increasing the sensitivity of mutation detection in different settings including cancer diagnostics and prenatal ...
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