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生物通
25 天
基于ITS序列的物种特异性PCR技术在柴胡药材及其常见伪品鉴定中的 ...
本研究通过开发靶向ITS区的物种特异性PCR技术,成功实现了《中国药典》正品柴胡(北柴胡Bupleurum chinense和南柴胡B. scorzonerifolium)与常见伪品(窄竹叶柴胡B. marginatum var. stenophyllum及锥叶柴胡B. bicaule)的精准鉴别。该方法可检出低至0.1 ng/μL的DNA模板,对人工掺伪 ...
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