Reverse Transcriptase | Function, Structure & Application

Reverse transcriptase (RT)-PCR: Principles, Applications • Microbe Online

Arrange the steps of diagnosing AIDS using the RT PCR method in the right order.I. DNA, engineered primers specific to HIV genes, and nucleotides along with buffers are incubated in a thermal cycler.II. Viral RNA is extracted from the patient’s plasma or blood sample.III. The PCR product is run on a gel to separate and identify the PCR amplified viral genes.IV. Reverse transcriptase is used to convert the RNA to cDNA.